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rat kidney proximal tubular epithelial cells  (ATCC)


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    ATCC rat kidney proximal tubular epithelial cells
    Effect of STS pre-treatment on cell death in an in vitro model of hypoxia–reoxygenation injury. Rat kidney <t>epithelial</t> <t>(NRK-52E)</t> cells were exposed to 24 h of 37 °C hypoxia in serum-free media followed by reoxygenation in serum-positive media for 24 h at 37 °C. Control cells were cultured in serum-positive media under normoxic conditions for the same period. Cells were stained with FITC-Annexin V (apoptosis) and propidium iodide (necrosis) for analysis via flow cytometry. Experimental cells received no treatment or were treated with 150 μM STS for 2 h before hypoxia (STS Pre), during hypoxia (STS Hyp), or both before and during hypoxia (STS Pre + Hyp). ( a ) Mean cell death percentage (FITC-Annexin + and/or PI + ). ( b ) Mean apoptosis percentage (FITC-Annexin + /PI − ). ( c ) Mean necrosis percentage (FITC-Annexin V − /PI + ). Bars represent mean ± SEM (n = 4). Means were compared using one-way ANOVA and Tukey’s post hoc test. *** p < 0.001, **** p < 0.0001. STS: sodium thiosulfate.
    Rat Kidney Proximal Tubular Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Effect of Sodium Thiosulfate Pre-Treatment on Renal Ischemia-Reperfusion Injury in Kidney Transplantation"

    Article Title: Effect of Sodium Thiosulfate Pre-Treatment on Renal Ischemia-Reperfusion Injury in Kidney Transplantation

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms25179529

    Effect of STS pre-treatment on cell death in an in vitro model of hypoxia–reoxygenation injury. Rat kidney epithelial (NRK-52E) cells were exposed to 24 h of 37 °C hypoxia in serum-free media followed by reoxygenation in serum-positive media for 24 h at 37 °C. Control cells were cultured in serum-positive media under normoxic conditions for the same period. Cells were stained with FITC-Annexin V (apoptosis) and propidium iodide (necrosis) for analysis via flow cytometry. Experimental cells received no treatment or were treated with 150 μM STS for 2 h before hypoxia (STS Pre), during hypoxia (STS Hyp), or both before and during hypoxia (STS Pre + Hyp). ( a ) Mean cell death percentage (FITC-Annexin + and/or PI + ). ( b ) Mean apoptosis percentage (FITC-Annexin + /PI − ). ( c ) Mean necrosis percentage (FITC-Annexin V − /PI + ). Bars represent mean ± SEM (n = 4). Means were compared using one-way ANOVA and Tukey’s post hoc test. *** p < 0.001, **** p < 0.0001. STS: sodium thiosulfate.
    Figure Legend Snippet: Effect of STS pre-treatment on cell death in an in vitro model of hypoxia–reoxygenation injury. Rat kidney epithelial (NRK-52E) cells were exposed to 24 h of 37 °C hypoxia in serum-free media followed by reoxygenation in serum-positive media for 24 h at 37 °C. Control cells were cultured in serum-positive media under normoxic conditions for the same period. Cells were stained with FITC-Annexin V (apoptosis) and propidium iodide (necrosis) for analysis via flow cytometry. Experimental cells received no treatment or were treated with 150 μM STS for 2 h before hypoxia (STS Pre), during hypoxia (STS Hyp), or both before and during hypoxia (STS Pre + Hyp). ( a ) Mean cell death percentage (FITC-Annexin + and/or PI + ). ( b ) Mean apoptosis percentage (FITC-Annexin + /PI − ). ( c ) Mean necrosis percentage (FITC-Annexin V − /PI + ). Bars represent mean ± SEM (n = 4). Means were compared using one-way ANOVA and Tukey’s post hoc test. *** p < 0.001, **** p < 0.0001. STS: sodium thiosulfate.

    Techniques Used: In Vitro, Control, Cell Culture, Staining, Flow Cytometry



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    Effect of STS pre-treatment on cell death in an in vitro model of hypoxia–reoxygenation injury. Rat kidney <t>epithelial</t> <t>(NRK-52E)</t> cells were exposed to 24 h of 37 °C hypoxia in serum-free media followed by reoxygenation in serum-positive media for 24 h at 37 °C. Control cells were cultured in serum-positive media under normoxic conditions for the same period. Cells were stained with FITC-Annexin V (apoptosis) and propidium iodide (necrosis) for analysis via flow cytometry. Experimental cells received no treatment or were treated with 150 μM STS for 2 h before hypoxia (STS Pre), during hypoxia (STS Hyp), or both before and during hypoxia (STS Pre + Hyp). ( a ) Mean cell death percentage (FITC-Annexin + and/or PI + ). ( b ) Mean apoptosis percentage (FITC-Annexin + /PI − ). ( c ) Mean necrosis percentage (FITC-Annexin V − /PI + ). Bars represent mean ± SEM (n = 4). Means were compared using one-way ANOVA and Tukey’s post hoc test. *** p < 0.001, **** p < 0.0001. STS: sodium thiosulfate.
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    Effect of STS pre-treatment on cell death in an in vitro model of hypoxia–reoxygenation injury. Rat kidney epithelial (NRK-52E) cells were exposed to 24 h of 37 °C hypoxia in serum-free media followed by reoxygenation in serum-positive media for 24 h at 37 °C. Control cells were cultured in serum-positive media under normoxic conditions for the same period. Cells were stained with FITC-Annexin V (apoptosis) and propidium iodide (necrosis) for analysis via flow cytometry. Experimental cells received no treatment or were treated with 150 μM STS for 2 h before hypoxia (STS Pre), during hypoxia (STS Hyp), or both before and during hypoxia (STS Pre + Hyp). ( a ) Mean cell death percentage (FITC-Annexin + and/or PI + ). ( b ) Mean apoptosis percentage (FITC-Annexin + /PI − ). ( c ) Mean necrosis percentage (FITC-Annexin V − /PI + ). Bars represent mean ± SEM (n = 4). Means were compared using one-way ANOVA and Tukey’s post hoc test. *** p < 0.001, **** p < 0.0001. STS: sodium thiosulfate.

    Journal: International Journal of Molecular Sciences

    Article Title: Effect of Sodium Thiosulfate Pre-Treatment on Renal Ischemia-Reperfusion Injury in Kidney Transplantation

    doi: 10.3390/ijms25179529

    Figure Lengend Snippet: Effect of STS pre-treatment on cell death in an in vitro model of hypoxia–reoxygenation injury. Rat kidney epithelial (NRK-52E) cells were exposed to 24 h of 37 °C hypoxia in serum-free media followed by reoxygenation in serum-positive media for 24 h at 37 °C. Control cells were cultured in serum-positive media under normoxic conditions for the same period. Cells were stained with FITC-Annexin V (apoptosis) and propidium iodide (necrosis) for analysis via flow cytometry. Experimental cells received no treatment or were treated with 150 μM STS for 2 h before hypoxia (STS Pre), during hypoxia (STS Hyp), or both before and during hypoxia (STS Pre + Hyp). ( a ) Mean cell death percentage (FITC-Annexin + and/or PI + ). ( b ) Mean apoptosis percentage (FITC-Annexin + /PI − ). ( c ) Mean necrosis percentage (FITC-Annexin V − /PI + ). Bars represent mean ± SEM (n = 4). Means were compared using one-way ANOVA and Tukey’s post hoc test. *** p < 0.001, **** p < 0.0001. STS: sodium thiosulfate.

    Article Snippet: Since renal proximal tubular epithelial cells are particularly susceptible to IRI, rat kidney proximal tubular epithelial cells (NRK-52E, ATCC, USA) were used, which is also consistent with our in vivo rat model of kidney transplantation [ ].

    Techniques: In Vitro, Control, Cell Culture, Staining, Flow Cytometry